Cycloheximide inhibits DNA synthesis in SV40-transformed cells
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چکیده
منابع مشابه
Temperature dependence of strand separation of the DNA molecules containing integrated SV40 DNA in transformed cells.
It has previously been demonstrated that the DNA molecules containing a genetic marker from one region of a bacterial genome undergo complete strand separation at temperatures usually different from the molecules containing a genetic marker from another region of the genome. The experiments also showed that if a group of molecules undergo complete strand separation over a narrow temperature ran...
متن کاملDetection of Specific Antigen in Sv40-transformed Cells by Immunofluorescence
With an immunofluorescent technique involving the use of serum of hamsters with SV40 tumors, nuclear fluorescence was detected in each of five cell lines, derived from four mammalian species, transformed by SV40 virus. Essentially all nuclei, including those of multinuclear cells, were fluorescent-stainable. Serum of hamsters bearing SV40 tumors was also found to give nuclear fluorescence in su...
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Heat shock factor 1 (HSF1), a main regulator of the heat shock response in eukaryotes, increases cell survival in numerous pathophysiological conditions. The aim of the present study was to o bserve the function of defective HSF1 expression in HEK293T cells. shRNA of human HSF1 was constructed into the retroviral vector pLTHR generating pLTHR‑shRNA‑HSF1. The shRNA was transiently transfected in...
متن کاملSV40 gene activity during lytic infection and in a series of SV40 transformed mouse cells.
Transcription of SV40 DNA was measured during lytic infection and in five SV40 transformed mouse cell lines. During productive infection, 50 per cent of viral DNA reacted with saturating amounts of lytic RNA. Varying portions of the SV40 genome were transcribed in transformed mouse cells, ranging from 15 per cent in a line which failed to yield virus, to 50 per cent in one line from which high ...
متن کاملEstablished lines of SV40-transformed human amnion cells.
Cells. Primary cultures of human amnion cells were prepared by trypsinizing amniotic membranes. Cultures were grown in Medium 5 12 (5) supplemented with 15% fetal bovine serum, penicillin (100 units/mi), and streptomycin (100 @ig/ml). Following SV4O infection, the cultures were maintained in McCoy's Medium SA (15) containing 10% agamma newborn calf serum. Approximately 6 weeks after infection, ...
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ژورنال
عنوان ژورنال: Nature
سال: 1981
ISSN: 0028-0836,1476-4687
DOI: 10.1038/291170a0